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Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M01432-2-carrier-free

Size: 100 µL

Boster Bio Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody catalog # M01432-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Keratin, type I cytoskeletal 14;Cytokeratin-14;CK-14;Keratin-14;K14;KRT14;

Gene Name

Keratin, type I cytoskeletal 14

UniProt

P02533

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Cytokeratin 14

Clonality

Monoclonal

Clone

Clone: FEF-11

Tissue Specificity

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. .

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51561 MW

Observed Molecular Weight

45 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

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