Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody catalog # M01262. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Synonyms

Melanocyte protein PMEL;ME20-M;ME20M;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;Premelanosome protein;Silver locus protein homolog;M-alpha;95 kDa melanocyte-specific secreted glycoprotein;P26;Secreted melanoma-associated ME20 antigen;ME20-S;ME20S;M-beta;PMEL;D12S53E, PMEL17, SILV;

Gene Name

Melanocyte protein PMEL

UniProt

P40967

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human PMEL17 / GP100

Clonality

Monoclonal

Clone

Clone: AHH-16

Tissue Specificity

Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

70255 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus. Melanosome. Endosome, multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.