Anti-Calreticulin Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Calreticulin Rabbit Monoclonal Antibody catalog # M00894. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Calreticulin;CRP55;Calregulin;Endoplasmic reticulum resident protein 60;ERp60;HACBP;grp60;CALR;CRTC;

Gene Name

Calreticulin

UniProt

P27797

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Calreticulin - ER Marker

Clonality

Monoclonal

Clone

Clone: CGO-3

Tissue Specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48142 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Endoplasmic reticulum lumen . Cytoplasm, cytosol . Secreted, extracellular space, extracellular matrix . Cell surface . Sarcoplasmic reticulum lumen . Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). Associated with the lytic granules in the cytolytic T-lymphocytes. .