Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody catalog # M00122-2. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Poly [ADP-ribose] polymerase 1;PARP-1;2.4.2.30;ADP-ribosyltransferase diphtheria toxin-like 1;ARTD1;NAD (+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1;PARP1;ADPRT, PPOL;

Gene Name

Poly [ADP-ribose] polymerase 1

UniProt

P09874

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Cleaved PARP

Clonality

Monoclonal

Clone

Clone: BHH-16

Tissue Specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .

Applications

WB,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to ly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. .