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Anti-Histone H2B Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M11068-carrier-free

Size: 100 µL

Boster Bio Anti-Histone H2B Rabbit Monoclonal Antibody catalog # M11068. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Histone H2B type 1-K;H2B K;HIRA-interacting protein 1;HIST1H2BK;H2BFT, HIRIP1;

Gene Name

Histone H2B type 1-K

UniProt

O60814

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Histone H2B

Clonality

Monoclonal

Clone

Clone: HAD-8

Tissue Specificity

Highly expressed in skeletal muscle, heart, liver and testis. .

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

13890 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:5000-1:20000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:30<br>FC 1:50

Subcellular Location

Nucleus. Chromosome.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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