Anti-CD3 epsilon Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-CD3 epsilon Rabbit Monoclonal Antibody catalog # M02675-2. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E;

Gene Name

T-cell surface glycoprotein CD3 epsilon chain

UniProt

P07766

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human CD3 epsilon

Clonality

Monoclonal

Clone

Clone: EED-3

Tissue Specificity

Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level). .

Applications

WB,IHC,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

The CD3 complex mediates signal transduction.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23147 MW

Observed Molecular Weight

250 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:30<br>FC 1:100

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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