Anti-GTPase HRAS Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-GTPase HRAS Rabbit Monoclonal Antibody catalog # M00114. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

GTPase HRas;H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras;GTPase HRas, N-terminally processed;HRAS;HRAS1;

Gene Name

GTPase HRas

UniProt

P01112

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human GTPase HRAS

Clonality

Monoclonal

Clone

Clone: BEA-8

Tissue Specificity

Widely expressed. .

Applications

WB,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21298 MW

Observed Molecular Weight

31 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:30

Subcellular Location

Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus. .