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Anti-Cdc16/APC6 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M04573-carrier-free

Size: 100 µL

Boster Bio Anti-Cdc16/APC6 Rabbit Monoclonal Antibody catalog # M04573. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Cell division cycle protein 16 homolog;Anaphase-promoting complex subunit 6;APC6;CDC16 homolog;CDC16Hs;Cyclosome subunit 6;CDC16;ANAPC6;

Gene Name

Cell division cycle protein 16 homolog

UniProt

Q13042

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Cdc16/APC6

Clonality

Monoclonal

Clone

Clone: IOI-3

Tissue Specificity

According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor- derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

Applications

WB,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71656 MW

Observed Molecular Weight

78 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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