Anti-Cyclin D3 CCND3 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Cyclin D3 CCND3 Rabbit Monoclonal Antibody catalog # M01744. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

G1/S-specific cyclin-D3;CCND3;

Gene Name

G1/S-specific cyclin-D3

UniProt

P30281

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Cyclin D3

Clonality

Monoclonal

Clone

Clone: GID-3

Tissue Specificity

Neutrophils.

Applications

WB,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32520 MW

Observed Molecular Weight

56 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>ICC/IF 1:50-1:200<br>FC 1:30

Subcellular Location

Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. .