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Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M00955-carrier-free

Size: 100 µL

Boster Bio Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody catalog # M00955. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP;HSPA5;GRP78;

Gene Name

78 kDa glucose-regulated protein

UniProt

P11021

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human GRP78 BiP

Clonality

Monoclonal

Clone

Clone: BOO-8

Tissue Specificity

Expressed in the excretory system, thyroid gland and Wilms tumors.

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72333 MW

Observed Molecular Weight

28 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Endoplasmic reticulum lumen. Melanosome. Cytoplasm . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

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