Anti-Vinculin VCL Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Vinculin VCL Rabbit Monoclonal Antibody catalog # M01207. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Vinculin;Metavinculin;MV;VCL;

Gene Name

Vinculin

UniProt

P18206

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Vinculin

Clonality

Monoclonal

Clone

Clone: AIG-22

Tissue Specificity

Metavinculin is muscle-specific.

Applications

IP,IF,IHC,ICC,WB

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:10000-1:20000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions. .