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Anti-Atg16L1/Atg16 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Atg16L1/Atg16 Rabbit Monoclonal Antibody catalog # M00526. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Autophagy-related protein 16-1;APG16-like 1;ATG16L1;APG16L;UNQ9393/PRO34307;

Gene Name

Autophagy-related protein 16-1

UniProt

Q676U5

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Atg16L1

Clonality

Monoclonal

Clone

Clone: ABIE-1

Tissue Specificity

Plasma. Synthesized by the fetal liver and yolk sac.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68265 MW

Observed Molecular Weight

40 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Cytoplasm . Preautophagosomal structure membrane ; Peripheral membrane protein . Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. .

Protein Name

T-cell surface glycoprotein CD4

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