Anti-hnRNP K Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-hnRNP K Rabbit Monoclonal Antibody catalog # M01793. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Heterogeneous nuclear ribonucleoprotein K;hnRNP K;Transformation up-regulated nuclear protein;TUNP;HNRNPK;HNRPK;

Gene Name

Heterogeneous nuclear ribonucleoprotein K

UniProt

P61978

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human hnRNP K

Clonality

Monoclonal

Clone

Clone: FOE-8

Tissue Specificity

Expressed in brain and lymphoblast.

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly (C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly (C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50976 MW

Observed Molecular Weight

40 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:5000-1:10000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:20

Subcellular Location

Cytoplasm . Nucleus, nucleoplasm . Cell projection, podosome . Recruited to p53/TP53- responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).