Anti-SNAP25 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-SNAP25 Rabbit Monoclonal Antibody catalog # M01625. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Synaptosomal-associated protein 25;SNAP-25;Super protein;SUP;Synaptosomal-associated 25 kDa protein;SNAP25;SNAP;

Gene Name

Synaptosomal-associated protein 25

UniProt

P60880

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human SNAP25

Clonality

Monoclonal

Clone

Clone: ABHE-19

Tissue Specificity

Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23315 MW

Observed Molecular Weight

43 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm, perinuclear region . Cell membrane ; Lipid-anchor. Cell junction, synapse, synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). .