Anti-Drosha Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Drosha Rabbit Monoclonal Antibody catalog # M00111. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Ribonuclease 3;3.1.26.3 ;Protein Drosha ;Ribonuclease III;RNase III;p241;DROSHA;RN3, RNASE3L, RNASEN;

Gene Name

Ribonuclease 3

UniProt

Q9NRR4

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Drosha

Clonality

Monoclonal

Clone

Clone: ABEF-4

Tissue Specificity

Ubiquitous. .

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

159316 MW

Observed Molecular Weight

159 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Nucleus . Nucleus, nucleolus . A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies. .