Anti-PABPN1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-PABPN1 Rabbit Monoclonal Antibody catalog # M02445. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Polyadenylate-binding protein 2;PABP-2;Poly (A)-binding protein 2;Nuclear poly (A)-binding protein 1;Poly (A)-binding protein II;PABII;Polyadenylate-binding nuclear protein 1;PABPN1;PAB2, PABP2;

Gene Name

Polyadenylate-binding protein 2

UniProt

Q86U42

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human PABPN1

Clonality

Monoclonal

Clone

Clone: AAHI-16

Tissue Specificity

Ubiquitous.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly (A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly (A) polymerase (PAPOLA) conferring processivity on the poly (A) tail elongation reaction and controls also the poly (A) tail length. Increases the affinity of poly (A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly (A) and to poly (G) with high affinity. May protect the poly (A) tail from degradation (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32749 MW

Observed Molecular Weight

53 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Nucleus . Cytoplasm . Nucleus speckle . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363). Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP- sensitive import mechanism (By similarity). Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly (A) RNA in nuclear speckles (By similarity). Intranuclear filamentous inclusions or "aggregates" are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly (A) RNA. .