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Anti-Cdc25B Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Cdc25B Rabbit Monoclonal Antibody catalog # M01899. Tested in WB, IP applications. This antibody reacts with Human.

Product Specifications

Synonyms

M-phase inducer phosphatase 2;3.1.3.48;Dual specificity phosphatase Cdc25B;CDC25B;CDC25HU2;

Gene Name

M-phase inducer phosphatase 2

UniProt

P30305

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Cdc25B

Clonality

Monoclonal

Clone

Clone: HIH-3

Tissue Specificity

Widely expressed (PubMed:16044242). Highly expressed in heart and skeletal muscle (PubMed:12802337). Expressed at intermediate level in brain, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes (PubMed:12802337). Weakly expressed in colon, thymus and small intestine (PubMed:12802337). .

Applications

WB,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. ly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

64987 MW

Observed Molecular Weight

270 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

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