Anti-BACE1/Bace Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-BACE1/Bace Rabbit Monoclonal Antibody catalog # M00322. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Beta-secretase 1;3.4.23.46;Aspartyl protease 2;ASP2;Asp 2;Beta-site amyloid precursor protein cleaving enzyme 1;Beta-site APP cleaving enzyme 1;Memapsin-2;Membrane-associated aspartic protease 2;BACE1;BACE, KIAA1149;

Gene Name

Beta-secretase 1

UniProt

P56817

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human BACE1

Clonality

Monoclonal

Clone

Clone: ABFF-2

Tissue Specificity

Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. .

Applications

WB,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

55711 MW

Observed Molecular Weight

47 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:40

Subcellular Location

Membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network . Endoplasmic reticulum. Endosome . Cell surface. Cytoplasmic vesicle membrane. Membrane raft . Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.