Anti-RPE65 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-RPE65 Rabbit Monoclonal Antibody catalog # M00824. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Retinoid isomerohydrolase;3.1.1.64;All-trans-retinyl-palmitate hydrolase;Retinal pigment epithelium-specific 65 kDa protein;Retinol isomerase;RPE65;

Gene Name

Retinoid isomerohydrolase

UniProt

Q16518

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human RPE65

Clonality

Monoclonal

Clone

Clone: ABAH-18

Tissue Specificity

Retinal pigment epithelium specific. .

Applications

WB,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60948 MW

Observed Molecular Weight

95 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50

Subcellular Location

Cytoplasm . Cell membrane ; Lipid-anchor . Microsome membrane . Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated (By similarity). Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells. .