Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody catalog # M01032. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Product Specifications

Synonyms

Hepatocyte nuclear factor 3-beta;HNF-3-beta;HNF-3B;Forkhead box protein A2;Transcription factor 3B;TCF-3B;FOXA2;HNF3B, TCF3B;

Gene Name

Hepatocyte nuclear factor 3-beta

UniProt

Q9Y261

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human FOXA2

Clonality

Monoclonal

Clone

Clone: AAAH-6

Tissue Specificity

Ubiquitous. .

Applications

WB,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48306 MW

Observed Molecular Weight

120 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>ICC/IF 1:50-1:200

Subcellular Location

Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.