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Anti-NEDD8 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-NEDD8 Rabbit Monoclonal Antibody catalog # M00547. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

NEDD8;Neddylin;Neural precursor cell expressed developmentally down-regulated protein 8;NEDD-8;Ubiquitin-like protein Nedd8;NEDD8;

Gene Name

NEDD8

UniProt

Q15843

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human NEDD8

Clonality

Monoclonal

Clone

Clone: AOAE-14

Tissue Specificity

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. .

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

9072 MW

Observed Molecular Weight

42 kDa, 48 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:20

Subcellular Location

Nucleus . Mainly nuclear.

Protein Name

T-cell surface glycoprotein CD4

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