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Anti-EBP50 SLC9A3R1 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M02427-carrier-free

Size: 100 µL

Boster Bio Anti-EBP50 SLC9A3R1 Rabbit Monoclonal Antibody catalog # M02427. Tested in WB, IHC applications. This antibody reacts with Human, Rat.

Product Specifications

Synonyms

Na (+)/H (+) exchange regulatory cofactor NHE-RF1;NHERF-1;Ezrin-radixin-moesin-binding phosphoprotein 50;EBP50;Regulatory cofactor of Na (+)/H (+) exchanger;Sodium-hydrogen exchanger regulatory factor 1;Solute carrier family 9 isoform A3 regulatory factor 1;SLC9A3R1;NHERF, NHERF1;

Gene Name

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

UniProt

O14745

Host

Rabbit

Reactivity

Human,Rat

Immunogen

A synthesized peptide derived from human EBP50

Clonality

Monoclonal

Clone

Clone: IIH-19

Tissue Specificity

Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. .

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38868 MW

Observed Molecular Weight

80 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Cytoplasm . Apical cell membrane . Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T- cells. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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