Anti-FoxO4/Afx Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-FoxO4/Afx Rabbit Monoclonal Antibody catalog # M01819. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Synonyms

Forkhead box protein O4;Fork head domain transcription factor AFX1;FOXO4;AFX, AFX1, MLLT7;

Gene Name

Forkhead box protein O4

UniProt

P98177

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human FoxO4

Clonality

Monoclonal

Clone

Clone: HEI-6

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53684 MW

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:5000-1:20000<br>IHC 1:50-1:200

Subcellular Location

Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.