Anti-Rad18 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Rad18 Rabbit Monoclonal Antibody catalog # M01622. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

E3 ubiquitin-protein ligase RAD18;6.3.2.-;Postreplication repair protein RAD18;hHR18;hRAD18;RING finger protein 73;RAD18;RNF73;

Gene Name

E3 ubiquitin-protein ligase RAD18

UniProt

Q9NS91

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Rad18

Clonality

Monoclonal

Clone

Clone: FFC-18

Tissue Specificity

Expressed ubiquitously in normal tissues.

Applications

WB,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56223 MW

Observed Molecular Weight

28 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:10000-1:20000<br>FC 1:20

Subcellular Location

Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565). .