Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody catalog # M00677. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1;3.1.4.11;PLC-148;Phosphoinositide phospholipase C-gamma-1;Phospholipase C-II;PLC-II;Phospholipase C-gamma-1;PLC-gamma-1;PLCG1;PLC1;

Gene Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

UniProt

P19174

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human PLCG1

Clonality

Monoclonal

Clone

Clone: FAA-16

Tissue Specificity

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

148532 MW

Observed Molecular Weight

149 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cell projection, lamellipodium. Cell projection, ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.