Anti-HTRA2/Omi Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-HTRA2/Omi Rabbit Monoclonal Antibody catalog # M01941. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Serine protease HTRA2, mitochondrial;3.4.21.108;High temperature requirement protein A2;HtrA2;Omi stress-regulated endoprotease;Serine protease 25;Serine proteinase OMI;HTRA2;OMI, PRSS25;

Gene Name

Serine protease HTRA2, mitochondrial

UniProt

O43464

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human HTRA2

Clonality

Monoclonal

Clone

Clone: EEH-8

Tissue Specificity

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

Applications

WB,IHC,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48841 MW

Observed Molecular Weight

28 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>IP 1:30

Subcellular Location

Mitochondrion intermembrane space. Mitochondrion membrane ; Single-pass membrane protein . Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.