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Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

M30950-1-carrier-free

Size: 100 µL

Boster Bio Anti-EpCAM/Trop1 Rabbit Monoclonal Antibody catalog # M30950-1. Tested in WB IHC, ICC/IF application. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Epithelial cell adhesion molecule;Ep-CAM;Adenocarcinoma-associated antigen;Cell surface glycoprotein Trop-1;Epithelial cell surface antigen;Epithelial glycoprotein;EGP;Epithelial glycoprotein 314;EGP314;hEGP314;KS 1/4 antigen;KSA;Major gastrointestinal tumor-associated protein GA733-2;Tumor-associated calcium signal transducer 1;CD326;EPCAM;GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1;

Gene Name

Epithelial cell adhesion molecule

UniProt

P16422

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from <a href="https://www.bosterbio.com/anti-ep-cam-cd326-epithelial-marker-antibody-m30941-boster.html" style="color:#EA8D28">human EpCAM</a>

Clonality

Monoclonal

Clone

Clone: CIB-5

Tissue Specificity

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Applications

WB,IHC,ICC/IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34932 MW

Observed Molecular Weight

50 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IHC: 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .

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