Anti-Rad51 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Rad51 Rabbit Monoclonal Antibody catalog # M00088. Tested in WB, Flow Cytometry, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

DNA repair protein RAD51 homolog 1;HsRAD51;hRAD51;RAD51 homolog A;RAD51;RAD51A, RECA;

Gene Name

DNA repair protein RAD51 homolog 1

UniProt

Q06609

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Rad51

Clonality

Monoclonal

Clone

Clone: CAE-18

Tissue Specificity

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Applications

WB,Flow Cytometry,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36966 MW

Observed Molecular Weight

75 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50<br>FC 1:50

Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.