Anti-Smad1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Smad1 Rabbit Monoclonal Antibody catalog # M00728-1. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Mothers against decapentaplegic homolog 1;MAD homolog 1;Mothers against DPP homolog 1;JV4-1;Mad-related protein 1;SMAD family member 1;SMAD 1;Smad1;hSMAD1;Transforming growth factor-beta-signaling protein 1;BSP-1;SMAD1;BSP1, MADH1, MADR1;

Gene Name

Mothers against decapentaplegic homolog 1

UniProt

Q15797

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Smad1

Clonality

Monoclonal

Clone

Clone: CAC-19

Tissue Specificity

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

Applications

WB,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52260 MW

Observed Molecular Weight

90 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.