Anti-Smad5 Rabbit Monoclonal Antibody, Carrier-free
Boster Bio Anti-Smad5 Rabbit Monoclonal Antibody catalog # M01423. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Synonyms
Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5;
Gene Name
Mothers against decapentaplegic homolog 5
UniProt
Q99717
Host
Rabbit
Reactivity
Human,Mouse,Rat
Immunogen
A synthesized peptide derived from human Smad5
Clonality
Monoclonal
Clone
Clone: BDB-19
Tissue Specificity
Ubiquitous.
Applications
WB,IHC,ICC,IF,Flow Cytometry
Purification
Affinity-chromatography
Concentration
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form
Liquid
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Components
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Storage Conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
52258 MW
Observed Molecular Weight
250 kDa
Fragment
Rabbit IgG
Applications Notes
WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50
Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Protein Name
Glycogen synthase kinase-3 beta (gsk3b)
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