Anti-Bcl-2 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio <a href="https://www.bosterbio.com/anti-bcl-2-antibody-monoclonal-ma1004-boster.html" style="color:#EA8D28">Anti-Bcl-2</a> Rabbit Monoclonal Antibody catalog # M00040-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Apoptosis regulator Bcl-2;BCL2;

Gene Name

Apoptosis regulator Bcl-2

UniProt

P10415

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Bcl-2

Clonality

Monoclonal

Clone

Clone: HF-2

Tissue Specificity

Expressed in a variety of tissues.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26266 MW

Observed Molecular Weight

83, 88 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:100<br>ICC/IF 1:50-1:100<br>IP 1:50<br>FC 1:50

Subcellular Location

Mitochondrion outer membrane ; Single-pass membrane protein . Nucleus membrane ; Single-pass membrane protein . Endoplasmic reticulum membrane ; Single-pass membrane protein .