Anti-TLR9 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-TLR9 Rabbit Monoclonal Antibody catalog # M00198. Tested in WB application. This antibody reacts with Human.

Product Specifications

Synonyms

Toll-like receptor 9;CD289;TLR9;UNQ5798/PRO19605;

Gene Name

Toll-like receptor 9

UniProt

Q9NR96

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human TLR9

Clonality

Monoclonal

Clone

Clone: ABFH-20

Tissue Specificity

Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.

Applications

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

115860 MW

Observed Molecular Weight

103 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000

Subcellular Location

Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endosome . Lysosome . Cytoplasmic vesicle, phagosome . Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. .