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Anti-NEFM/Nf M Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-NEFM/Nf M Rabbit Monoclonal Antibody catalog # M06821. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Product Specifications

Synonyms

Neurofilament medium polypeptide;NF-M;160 kDa neurofilament protein;Neurofilament 3;Neurofilament triplet M protein;NEFM;NEF3, NFM;

Gene Name

Neurofilament medium polypeptide

UniProt

P07197

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human NEFM

Clonality

Monoclonal

Clone

Clone: AAGI-14

Tissue Specificity

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. .

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

102472 MW

Observed Molecular Weight

94 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:20

Subcellular Location

Nucleus. Chromosome.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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