Anti-CD74 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-CD74 Rabbit Monoclonal Antibody catalog # M01340-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

HLA class II histocompatibility antigen gamma chain;HLA-DR antigens-associated invariant chain;Ia antigen-associated invariant chain;Ii;p33;CD74;CD74;DHLAG;

Gene Name

HLA class II histocompatibility antigen gamma chain

UniProt

P04233

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human CD74

Clonality

Monoclonal

Clone

Clone: AOIF-3

Tissue Specificity

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and ing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33516 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Cell membrane ; Single-pass type II membrane protein . Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.