Anti-TRF1 TERF1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-TRF1 TERF1 Rabbit Monoclonal Antibody catalog # M02474. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Telomeric repeat-binding factor 1;NIMA-interacting protein 2;TTAGGG repeat-binding factor 1;Telomeric protein Pin2/TRF1;TERF1;PIN2, TRBF1, TRF, TRF1;

Gene Name

Telomeric repeat-binding factor 1

UniProt

P54274

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human TRF1

Clonality

Monoclonal

Clone

Clone: AOIB-20

Tissue Specificity

Highly expressed and ubiquitous. Isoform Pin2 predominates.

Applications

WB,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double- stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50246 MW

Observed Molecular Weight

48 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, telomere. Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.