Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody catalog # M00121. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB;

Gene Name

Fibroblast growth factor 2

UniProt

P09038

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human FGF2

Clonality

Monoclonal

Clone

Clone: AOHC-6

Tissue Specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .

Applications

WB,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30770 MW

Observed Molecular Weight

70 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50<br>FC 1:50

Subcellular Location

Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.