Anti-TRX1 TXN Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-TRX1 TXN Rabbit Monoclonal Antibody catalog # M01219-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Thioredoxin;Trx;ATL-derived factor;ADF;Surface-associated sulphydryl protein;SASP;TXN;TRDX, TRX, TRX1;

Gene Name

Thioredoxin

UniProt

P10599

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human TRX1

Clonality

Monoclonal

Clone

Clone: AOGB-20

Tissue Specificity

Highly expressed in the corneal epithelium. .

Applications

WB,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

11737 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:5000<br>FC 1:100

Subcellular Location

Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.