Anti-ARF6 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-ARF6 Rabbit Monoclonal Antibody catalog # M00927. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

ADP-ribosylation factor 6;ARF6;

Gene Name

ADP-ribosylation factor 6

UniProt

P62330

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human ARF6

Clonality

Monoclonal

Clone

Clone: AOFF-1

Tissue Specificity

Ubiquitous, with higher levels in heart, substantia nigra, and kidney. .

Applications

WB,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

20082 MW

Observed Molecular Weight

120-150 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200

Subcellular Location

Golgi apparatus. Cell membrane; Lipid- anchor. Endosome membrane; Lipid-anchor. Recycling endosome membrane ; Lipid-anchor . Cell projection, filopodium membrane; Lipid-anchor. Midbody . Cytoplasm . Cleavage furrow . Distributed throughout the cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis. Recruited to the midbody at later stages of cytokinesis; this requires interaction with KIF23 (By similarity). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles. .