Anti-MiTF Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-MiTF Rabbit Monoclonal Antibody catalog # M00269-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32;MITF;BHLHE32;

Gene Name

Microphthalmia-associated transcription factor

UniProt

O75030

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human MiTF

Clonality

Monoclonal

Clone

Clone: AOED-13

Tissue Specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines. .

Applications

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'- CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. .