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Anti-iNOS NOS2 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-iNOS NOS2 Rabbit Monoclonal Antibody catalog # M00368. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

Nitric oxide synthase, inducible;1.14.13.39;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;N

Gene Name

Nitric oxide synthase, inducible

UniProt

P35228

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human iNOS

Clonality

Monoclonal

Clone

Clone: IED-14

Tissue Specificity

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS- S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys- 247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

131117 MW

Observed Molecular Weight

150 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cell membrane ; Single- pass type I membrane protein . The activated receptor is rapidly internalized and degraded.

Protein Name

T-cell surface glycoprotein CD4

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