Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-LC3B MAP1LC3B Rabbit Monoclonal Antibody catalog # M01524. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Microtubule-associated proteins 1A/1B light chain 3B;Autophagy-related protein LC3 B;Autophagy-related ubiquitin-like modifier LC3 B;MAP1 light chain 3-like protein 2;MAP1A/MAP1B light chain 3 B;MAP1A/MAP1B LC3 B;Microtubule-associated protein 1 light chain 3 beta;MAP1LC3B;MAP1ALC3;

Gene Name

Microtubule-associated proteins 1A/1B light chain 3B

UniProt

Q9GZQ8

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human LC3B

Clonality

Monoclonal

Clone

Clone: IEC-13

Tissue Specificity

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. .

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

14688 MW

Observed Molecular Weight

43 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome . LC3-II binds to the autophagic membranes. Localizes also to discrete punctae along the ciliary axoneme (By similarity). .