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Anti-MGMT Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-MGMT Rabbit Monoclonal Antibody catalog # M01012. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Synonyms

Methylated-DNA--protein-cysteine methyltransferase;2.1.1.63;6-O-methylguanine-DNA methyltransferase;MGMT;O-6-methylguanine-DNA-alkyltransferase;MGMT;

Gene Name

Methylated-DNA--protein-cysteine methyltransferase

UniProt

P16455

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human MGMT

Clonality

Monoclonal

Clone

Clone: IDD-13

Tissue Specificity

Plasma.

Applications

WB,IHC

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21646 MW

Observed Molecular Weight

22 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200

Subcellular Location

Nucleus.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

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