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Anti-E2F2 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-E2F2 Rabbit Monoclonal Antibody catalog # M02896. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Transcription factor E2F2;E2F-2;E2F2;

Gene Name

Transcription factor E2F2

UniProt

Q14209

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human E2F2

Clonality

Monoclonal

Clone

Clone: GGC-5

Tissue Specificity

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Applications

WB,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

47506 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>FC 1:100

Subcellular Location

Nucleus.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Curated Selection

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