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Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody catalog # M00174. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.

Product Specifications

Synonyms

POU domain, class 5, transcription factor 1;Octamer-binding protein 3;Oct-3;Octamer-binding protein 4;Oct-4;Octamer-binding transcription factor 3;OTF-3;POU5F1;OCT3, OCT4, OTF3;

Gene Name

POU domain, class 5, transcription factor 1

UniProt

Q01860

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Oct4

Clonality

Monoclonal

Clone

Clone: GDI-16

Tissue Specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. .

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

38571 MW

Observed Molecular Weight

29 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm. Nucleus. Expressed in a diffuse and slightly punctuate pattern.

Protein Name

P2X purinoceptor 1

Curated Selection

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