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Anti-CD45 PTPRC Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-CD45 PTPRC Rabbit Monoclonal Antibody catalog # M00555-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45;

Gene Name

Receptor-type tyrosine-protein phosphatase C

UniProt

P08575

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human CD45

Clonality

Monoclonal

Clone

Clone: CBI-16

Tissue Specificity

Ubiquitous.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

147254 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:100<br>FC 1:20

Subcellular Location

Membrane ; Single-pass type I membrane protein . Membrane raft . Colocalized with DPP4 in membrane rafts.

Protein Name

T-cell surface glycoprotein CD4

Curated Selection

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