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Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-ENO1/Alpha Enolase Rabbit Monoclonal Antibody catalog # M01250. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Alpha-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;C-myc promoter-binding protein;Enolase 1;MBP-1;MPB-1;Non-neural enolase;NNE;Phosphopyruvate hydratase;Plasminogen-binding protein;ENO1;ENO1L1, MBPB1, MPB1;

Gene Name

Alpha-enolase

UniProt

P06733

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human ENO1

Clonality

Monoclonal

Clone

Clone: AEA-5

Tissue Specificity

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

47169 MW

Observed Molecular Weight

80 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm . Cell membrane . Cytoplasm, myofibril, sarcomere, M line . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Frequently Asked Questions

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