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Anti-Bad Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Bad Rabbit Monoclonal Antibody catalog # M03520. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Bcl2-associated agonist of cell death;BAD;Bcl-2-binding component 6;Bcl-2-like protein 8;Bcl2-L-8;Bcl-xL/Bcl-2-associated death promoter;Bcl2 antagonist of cell death;BAD;BBC6, BCL2L8;

Gene Name

Bcl2-associated agonist of cell death

UniProt

Q92934

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Bad

Clonality

Monoclonal

Clone

Clone: CHC-2

Tissue Specificity

Expressed in a wide variety of tissues.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X (L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

18392 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:30<br>FC 1:50

Subcellular Location

Mitochondrion outer membrane. Cytoplasm . Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm. .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

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