Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody, Carrier-free
Boster Bio Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody catalog # P01423-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Product Specifications
Phospho Site
S463
Synonyms
Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5;SMAD5;MADH5;
Gene Name
Mothers against decapentaplegic homolog 5
UniProt
Q99717
Host
Rabbit
Reactivity
Human,Mouse,Rat
Immunogen
A synthesized peptide derived from human Phospho-Smad5 (S463/465)
Clonality
Monoclonal
Clone
Clone: AFB-19
Tissue Specificity
Ubiquitous.
Applications
WB,IHC,ICC,IF
Purification
Affinity-chromatography
Concentration
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form
Liquid
Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Components
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Storage Conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
52258 MW
Observed Molecular Weight
130 kDa
Fragment
Rabbit IgG
Applications Notes
WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200
Subcellular Location
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
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