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Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

P00235-carrier-free

Size: 100 µL

Boster Bio Anti-Phospho-Vimentin (S72) Rabbit Monoclonal Antibody catalog # P00235. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Phospho Site

S72

Synonyms

Vimentin;VIM;

Gene Name

Vimentin

UniProt

P08670

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Phospho-Vimentin (S72)

Clonality

Monoclonal

Clone

Clone: ADE-22

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .

Applications

WB,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53652 MW

Observed Molecular Weight

100-110 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50

Subcellular Location

Cytoplasm .

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