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Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

P00075-carrier-free

Size: 100 µL

Boster Bio Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody catalog # P00075. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Phospho Site

T401

Synonyms

Serine/threonine-protein kinase B-raf;2.7.11.1;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1;BRAF;BRAF1, RAFB1;

Gene Name

Serine/threonine-protein kinase B-raf

UniProt

P15056

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Phospho-B Raf (T401)

Clonality

Monoclonal

Clone

Clone: IBE-2

Tissue Specificity

Brain and testis.

Applications

WB,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

84437 MW

Observed Molecular Weight

90-100 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>IP 1:30

Subcellular Location

Nucleus . Cytoplasm. Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .

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